Journal: Journal of Virology

Article Title: Early Transcriptional Changes within Liver, Adrenal Gland, and Lymphoid Tissues Significantly Contribute to Ebola Virus Pathogenesis in Cynomolgus Macaques

doi: 10.1128/JVI.00250-20

Figure Lengend Snippet: Antibodies used for flow cytometric analysis of immune cell subsets a

Article Snippet: Gating strategies and antibody information were previously described ( 20 ) ( ). table ft1 table-wrap mode="anchored" t5 TABLE 3 caption a7 Flow panels Antibody Clone b Company b Catalog no. b Monocyte/DC basic Mouse anti-human CD3 FITC SP34-2 BD 556611 Mouse anti-human CD20 FITC 2H7 BD 556632 Mouse anti-human CD16 PE 3G8 BioLegend 302008 Mouse anti-human CD11c PE-Cy7 3.9 BioLegend 301608 Mouse anti-human CD14 APC M5E2 BioLegend 301808 Mouse anti-human CD123 PerCP-Cy5.5 6H6 BioLegend 306016 Mouse anti-human HLA-DR APC-Cy7 L243 BioLegend 307618 NK Mouse anti-human CD3 FITC – – – Mouse anti-human CD20 FITC – – – Mouse anti-human CD14 FITC M5E2 BioLegend 301804 Mouse anti-human CD56 PE-Cy7 B159 BD 557747 Mouse anti-human granzyme B Alex647* GB11 BioLegend 515405 Mouse anti-human CD159a PE Z199 Beckman Coulter IM3291U Mouse anti-human CD8a APC-Cy7 RPA-T8 BioLegend 301016 Mouse anti-human CD16 PerCP-Cy5.5 3G8 BioLegend 302028 DC Activation Mouse anti-human CD3 FITC – – – Mouse anti-human CD20 FITC – – – Mouse anti-human CD14 FITC M5E2 BioLegend 301804 Mouse anti-human HLA-DR APC-Cy7 – – – Mouse anti-human CD11c PE-Cy7 – – – Mouse anti-human CD123 PerCpCy5.5 – – – Mouse anti-human CD1c APC AD5-8E7 Miltenyi 130-090-903 Mouse anti-human CD86 PE IT2.2 BioLegend 305406 T cells Mouse anti-human CD4 PerCp-Cy5.5 OKT4 Tonbo 65-0048 Mouse anti-human CD8b PE 2ST8.5H7 BD 641057 Mouse anti-human CD28 PE-Cy7 CD28.2 Tonbo 60-0289 Mouse anti-human CD95 APC DX2 BD 558814 Mouse anti-human CCR7 APC-Cy7 G043H7 BioLegend 353212 Mouse anti-human Ki67 FITC* B56 BD 556026 B cells Mouse anti-human CD20 PE-Cy7 2H7 eBioscience 25-0209-73 Mouse anti-human CD27 APC O323 Tonbo 20-0279 Mouse anti-human IgD PE IA6-2 Biolegend 348204 Mouse anti-human CD40 APC-Cy7 5C3 BioLegend 334324 Mouse anti-human Ki67 FITC* – – – Mouse anti-human IgG PerCP-Cy5.5 HP6017 BioLegend 409312 Open in a separate window a *Denotes intracellular antibody. b –, Same as monocyte/DC basic panel.

Techniques: Activation Assay

Journal: Mediators of Inflammation

Article Title: The Distribution of Activation Markers and Selectins on Peripheral T Lymphocytes in Preeclampsia

doi: 10.1155/2017/8045161

Figure Lengend Snippet: Prevalence of the investigated cell subsets.

Article Snippet: PBMCs were incubated for 30 min at room temperature in the dark with the following antibodies: APC-Cy7-conjugated CD4, PerCP-conjugated HLA-DR, APC-conjugated CD62E, PE-Cy7-conjugated CD62L, PE-conjugated CD62P, FITC-conjugated CD25, PE-Cy7-conjugated CD11c, PE-conjugated CD122, and APC-conjugated CD69 (BD Biosciences).

Techniques: Activation Assay